RBK Projects

Phase 2 Projects (Active)

Projects with a focus on Repair/Regeneration

• AKI Therapeutics
  Neil Hukriede (Contact PI), University of Pittsburgh; Alan Davidson, University of Auckland; Mark De Caestecker, Vanderbilt University; Donna Huryn, University of Pittsburgh
• Cell-specific Delivery of Novel Therapies to Enhance Glomerular Regeneration and Repair
  Stuart Shankland (Contact PI), University of Washington; Benjamin J. Freedman, University of Washington; James M. Roberts, Lumen Bioscience; Oliver Wessely, Cleveland Clinic
• Integrating UB collecting systems into hPSC-derived kidney organoids
  Kyle McCracken, Cincinnatti Children's
• Single-cell Analysis to Promote Kidney Repair
  Benjamin D. Humphreys (Contact PI), Washington University in St. Louis; Junhyong Kim, University of Pennsylvania; Andrew McMahon, University of Southern California
• Single-nuclei multiome atlas for a mammalian model of kidney regeneration
  Elizabeth Nguyen, Seattle Children's
• Stepwise developmental mimicry generates proximal tubules in organoids
  Nils Lindstrom, University of Southern California

Projects with a Focus on Building

• Building Extracellular Matrix Scaffolds to Induce Nephron Development
  Jason A. Wertheim, University of Arizona
• Engineering Functional Kidneys
  Jennifer Lewis (Contact PI), Harvard University; Ryuji Morizane, Harvard University; Lisa Satlin, Icahn School of Medicine
• Functional Renal Engraftment
  Iain Drummond (Contact PI), Mount Desert Island Biological Laboratory; Leif Oxburgh, The Rogosin Institute; Denise Marciano, University of Texas, Southwestern
• Kidney Microphysiological Analysis Platforms (MAP) to Optimize Function and Model Disease
  Joseph Bonventre (Contact PI), Brigham and Women's Hospital; Luke Lee, Brigham and Women's Hospital; Jennifer Lewis, Harvard University
• The Synthetic Kidney: A Revolutionary Solution for the Shortage of Kidneys for Transplantation
  Zhongwei Li, University of Southern California
• Visualizing Vascularization and Cell Interactions in Implanted Organoids
  Leif Oxburgh (Contact PI), The Rogosin Institute; Ondine Cleaver, University of Texas, Southwestern; Tom Carroll, University of Texas, Southwestern; Denise Marciano, University of Texas, Southwestern; Bradley Yoder, University of Alabama, Birmingham

Phase 1 Projects (Completed)

Bioengineering

• 3D Bioprinting of Vascularized, Convoluted Renal Proximal Tubules
  (Jennifer Lewis, Harvard University)

• Application of Progenitor Niche Signals to Ex Vivo Nephrogenesis
  (Leif Oxburgh, Maine Medical Center / Thomas Carroll, University of Texas Southwestern Medical Center / Ondine Cleaver, University of Texas Southwestern Medical Center / David Kaplan, Tufts University)

• Cytokines and Extracellular Matrix
  (Jeffrey A. Hubbell, University of Chicago)

• Rebuilding the Glomerular Filtration Barrier by Regenerating Adult Podocytes
  (Stuart J. Shankland, University of Washington / Ying Zheng, University of Washington)

Cell-type specific transcriptional profiling

• Cellular Diversity in Human Nephrogenesis
  (Andrew McMahon, University of Southern California)

• Identifying Mechanisms of Renal Repair
  (Andrew McMahon, University of Southern California)

• Transcriptional Profiling of In Vivo Derived Human Kidney Tissue Using Single Cell RNA Sequencing
  (Jason Spence, University of Michigan)

Coordinating Center

• ReBuilding the Kidney Coordinating Center
  (Carl Kesselman, University of Southern California)

Endogenous Repair

• Adult Progenitors in Kidney Tubulointerstitium
  (Benjamin D. Humphreys, Washington University in St. Louis)

Generation of complex kidney structures

• Generating an Interconnected Kidney Tubule Architecture
  (Iain A. Drummond, Mount Desert Island Biological Laboratory)

Growth and differentiation

• Differentiation of Human Pluripotent Stem Cells into Kidney Cell Lineages
  (Oliver Wessely, Cleveland Clinic / Jan Jensen, Cleveland Clinic)

Organoids

• Developing Pro-regenerative Drug Therapies for Acute Kidney Injury
  (Neil Hukriede, University of Pittsburgh / Alan Davidson, University of Auckland)

• Generating Kidney Organoids and Mature Renal Cell Types Via Directed iPSC Differentiation
  (Melissa H. Little, Murdoch Childrens Research Institute)

Physiology

• Mechanoregulation of Cation Transport in Bioprinted Collecting Ducts
  (Lisa Satlin, Icahn School of Medicine at Mount Sinai)

• Physiologic Properties of Human Kidney Organoids
  (Thomas Kleyman, University of Pittsburgh)

Protocols - Analysis of renal biopsies

• Human Kidney Biopsy Single Cell Protocols and Analysis
  (Benjamin D. Humphreys, Washington University in St. Louis)

• Identifying Kidney Cell Phenotype Factors Using Single Cell RNA Sequencing
  (Junhyong Kim, University of Pennsylvania / James Eberwine, University of Pennsylvania)

• Using Cold Active Proteases for Single Cell Dissociation
  (S. Steven Potter, Cincinnati Children's Hospital)

Protocols - Proteomic profiling

• Spatially-preserved Expression Analysis of Kidney Cells in Human Biopsy Tissue
  (Lloyd Cantley, Yale University)

iPSC reporter lines

• Differentiation of Fluorescent Reporter Human iPS Cell Lines for Monitoring Renal Cell Lineages
  (Todd Valerius, Brigham and Women's Hospital)

• Engineering Human Collecting System Reporter Pluripotent Stem Cell Lines
  (Sanjay Jain, Washington University in St. Louis)