Identifying Kidney Cell Phenotype Factors Using Single Cell RNA Sequencing

Key Personnel

Junhyong Kim (PI)
University of Pennsylvania

James Eberwine (PI)
University of Pennsylvania

• Susanna Nazarian
  University of Pennsylvania

Project Description

The goal of the Penn project is to develop molecular biology and informatics tools to identify key regulatory factors that determine kidney cell phenotypes. Single cell resolution transcriptome data can reveal cryptic cell types as well as help identify regulatory systems related to cell phenotypes. We hypothesize that cell phenotype determining genes are often at low to moderate abundance in a cell, making them difficult to approach using current methods. The Penn group will develop novel molecular biology techniques to enrich single cell transcriptomes for low abundance genes. We will also develop informatics tools that will help identify targets for trans-differentiation experiments, leveraging single cell level measurements.